High Security Animal Disease Laboratory

High Security Animal Disease Laboratory

Indian Veterinary Research Institute, Indian Council of Agricultural Research

Reference Laboratory for Avian Influenza

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Transmission Electron Microscope (TEM) Unit

Transmission Electron Microscope(TEM) Unit

The possibility for high magnifications and greater resolutions has made the Transmission Electron Microscope (TEM) a valuable tool in both biological and materials research. A TEM facility costing about Rs. 1.80 crores is available in a dedicated building at HSADL campus. This was made possible through a special grant sanctioned by Hon’ble Director General ICAR, Dr. Mangla Rai during Xth plan after the first outbreak of bird flu (Avian influenza HPAI-H5N1) in 2006. The unit was inaugurated on 31.07.2009. EM unit of HSADL has a 120 kV class high-resolution transmission electron microscope (JEM-1400, Jeol, Japan). It is fitted with a digital CCD camera that enables focusing and image verification on the screen, providing filmless recording of images. The unit also has an ultramicrotome (Ultracut-UCT, Leica) and a knife maker (EMKMR2, Leica) for ultra-thin sectioning of tissue samples. The services of the TEM unit add to the existing array of diagnostic facilities available at HSADL for quick confirmation of various exotic and emerging viral infections of animals. Apart from being useful in ultrastructural studies on viral infections also, it can be of great help in identification and confirmation of viral etiology in case of unknown disease outbreaks in future.

TEM at HSADL (Jeol JEM-1400)